Chronic Changes In Serum IL-6 And TNF-α Following 12 Weeks of Concurrent Resistance And Aerobic Exercise Are Dependent On Exercise Mode And May Affect Adaptation

Authors

Brad S. Lambert, Applied Exercise Science Laboratory, Texas A & M University - College StationFollow
Justin P. Dobson, Texas A&M University
Stephen F. Crouse, Texas A&M University

Abstract

PURPOSE: To examine physiological responses to concurrent resistance and land treadmill training (RT-LTM) compared to concurrent resistance and aquatic treadmill training (RT-ATM) and the chronic effect of each on serum TNF-α and IL-6 (cytokines associated with chronic inflammation, CVD, and skeletal muscle metabolism). METHODS: Twenty-six untrained subjects (M: n=13, 98.6±17.1kg, 182.2±6.2cm, 34±11yrs, F: n=13, 78.9±14.0kg, 165.1±5.1cm, 38±11yrs) were screened to assess VO_2max, body composition (DEXA), and strength (Lifts: leg press, chest press, leg curl, lat pull, leg ext, triceps push-down, biceps curl). Subjects were then randomized into 2 groups: RT-LTM (M=6, F=7), RT-ATM (M=7, F=6). Each performed progressive RT (2/wk, 3 x 8-12 @ 60%à~80% 1RM) for 12 wks. Both groups also performed 12 wks of aerobic LTM or ATM (60à85%VO_2max) respectively. ATM or LTM occurred immediately following RT sessions and in isolation on a 3^rd day during the wk. Kcal/session: Wk 1-6 = 250à500 kcal/session, Wk 6-12 = 500 kcal/session. Blood samples were obtained in the rested state (>72h after last exercise bout) before and after training. Serum TNF-α and IL-6 was analyzed using a multiplex assay kit (Luminex®, Millipore®). A 2x2 Mixed Model ANOVA w/ repeated measures was used to examine absolute and relative changes in the independent variables listed in the table. RESULTS:

INDEP. VAR.

Lean Mass (kg)

Fat Mass (kg)

%Body Fat (%)

VO_2max (ml/kg/min)

Total Strength (lbs)

IL-6 (pg/dl)

TNF-α (pg/dl)

BASELINE MEASUREMENTS

RT-LTM

49.9 ± 3.7

35.9 ± 3.1

42.1 ± 2.5

29.9 ± 2.0

1457.4 ± 135.1

4.6 ± 1.6

7.1 ± 1.9

RT-ATM

53.1 ± 4.0

31.1 ± 2.8

37.0 ± 2.3

32.1 ± 1.6

1552.8 ± 145.9

4.6 ± 1.5

7.9 ± 1.5

POST TRAINING MEASUREMENTS

RT-LTM

51.0 ± 4.7^†

34.1 ± 2.7^†

40.1 ± 3.6^†

35.8 ± 2.9^†

1843.4 ± 201.1^†

7.1 ± 1.6^†

6.8 ± 0.5

RT-ATM

55.8 ± 4.6^†

30.3 ± 3.1

35.6 ± 2.4^†

35.8 ± 2.3^†

2193.6 ± 251.3^†

5.1 ± 2.7

6.6 ± 1.0^†

%∆ = Calculated From Each Individual Subjects Change From Baseline

RT-LTM

2.6% ± 1.4^†,a

-6.5% ± 2.4^†,a

-5.8% ± 1.8^†,a

14.1% ± 2.3^†,a

21.3% ± 1.1^†,a

125.9% ± 36.6^†,a

-1.8% ± 6.0^a

RT-ATM

4.2% ± 0.9^†,b

-2.1% ± 1.6^b

-4.1% ± 1.5^†,a

4.5% ± 3.0^†,b

27.1% ± 1.7^†,b

28.1% ± 34.7^b

-13.0% ± 5.4^†,b

Values are means ± SE. %Δ = Individual change from baseline. Like letters = not significantly different between groups, †=Significant change from baseline within group (α ≤ 0.05).

CONCLUSION: Chronic RT-LTM and RT-ATM training elicit different effects on markers of chronic inflammation which may be related to differing health and fitness outcomes observed between our groups.

Recommended Citation

Lambert, Brad S.; Dobson, Justin P.; and Crouse, Stephen F. (2012) "Chronic Changes In Serum IL-6 And TNF-α Following 12 Weeks of Concurrent Resistance And Aerobic Exercise Are Dependent On Exercise Mode And May Affect Adaptation," International Journal of Exercise Science: Conference Proceedings: Vol. 2: Iss. 4, Article 11.
Available at: https://digitalcommons.wku.edu/ijesab/vol2/iss4/11