Department of Biology
Master of Science
Genetic suppressor elements (GSEs) are short biologically active gene fragments that encode inhibitory antisense RNAs or truncated proteins that function as negative dominants. GSEs can be constructed from a single gene or isolated from a multigene library consisting of short random fragments of the target gene or genes. The goal of this study was to determine if gene inhibition in bacterial cells could be achieved by isolating GSEs from a genomic library. Additionally, if it was possible to use GSEs to inhibit gene function in E. coli JM109DE3 cells, could this method be used to locate and determine the function of unknown genes? In this study, antisense GSEs were isolated clearly showing that it was possible to inhibit function of known and unknown genes in bacterial cells using genetic suppressor elements derived from a genomic library.
Chuke, Ronald, "Gene Inhibition Using Genetic Suppressor Elements in Escherichia Coli JM109DE3 Cells" (2000). Masters Theses & Specialist Projects. Paper 715.