J Barnes Benton, Daphney M. Stanford, Matthew A. Chatlaong, Matthew B. Jessee. University of Mississippi, University, MS.

BACKGROUND: While it is recommended that blood flow restriction is applied relative to pre-exercise (or resting) arterial occlusion pressure (AOP), there are no guidelines for an initial rest period prior to the measurement. Remeasuring AOP post-exercise can be used to estimate the cardiovascular response. When measuring post-exercise AOP, different methods have been used. Some have increased the cuff pressure from blood flow restriction exercise pressure (without deflation), and others deflate the cuff prior to post-exercise AOP measurement. It is unknown if pre-exercise AOP is affected by initial rest period, or if post-exercise AOP is affected by cuff deflation. Thus, the purpose of this study is to: 1) compare pre-exercise AOP across differing initial rest periods, and 2) compare post-exercise AOP measured either with or without cuff deflation following exercise. METHODS: We plan to recruit 50 participants for a total of three visits. Visit 1 will consist of paperwork and familiarization, followed by measurements of height, weight, and 1 repetition maximum (1RM) bicep curl testing on the upper dominant arm. Visits 2 and 3 will be exercise testing days. Upon entry into the lab on visits 2 and 3, AOP will be measured immediately. Depending on condition, AOP will be measured 5min after initial AOP. On both days, AOP will be measured 10min after initial measurement serving as pre-exercise AOP. Exercise will be performed as three sets of elbow flexion curls at 30% 1RM to failure and 40% of pre-exercise AOP with 30s rest between sets. 10s after the cessation of exercise, post-exercise AOP will be measured. Depending on condition, to assess AOP, the pressure will be increased from 40% AOP, or the cuff will be deflated and reinflated. To determine differences in post-exercise AOP, we will use a Bayesian two-way repeated measures ANOVA. For differences across rest periods, we will use a Bayesian one-way repeated measures ANOVA. EXPECTED RESULTS: For comparison of pre-exercise rest periods, we would expect no difference between the immediate, 5min, and 10min AOP measures. Due to the hyperemic response subsequent to the blood pooling effect created by blood flow restriction exercise, we hypothesize that cuff deflation directly post-exercise would have a greater change in AOP pre to post exercise. We would expect to see the number of completed repetitions to be greatest in set one and decrease with each set.

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