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AGE AND PHYSICAL ACTIVITY DO NOT IMPACT CIRCULATING SOLUBLE CD14 CONCENTRATION IN APPARENTLY HEALTHY ADULTS

Abstract

BACKGROUND: Soluble CD14 (sCD14) (cluster of differentiation 14) is a co-receptor of bacterial lipopolysaccharide that is released from monocytes upon activation. There is a well-recognized role of sCD14 in inflammation, and aging has been associated with increased inflammation and cardiometabolic health risk factors (e.g., high blood pressure [BP], hyperglycemia). However, there is limited information on the combined influence of aging and physical activity on circulating sCD14. Therefore, the purpose of this study was to examine the influence of aging on plasma sCD14 concentration and the potential influence of physical activity. METHODS: Twenty young (11 females, age 22.7± 2.6, body mass index 26.7 ± 3.3, BP 119/73 ± 7/8 mmHg) and 21 old (11 female, age 58.4 ± 7.4, body mass index 27.8 ± 4.8, BP 125/76 ± 12/8 mmHg) adults participated in the study. Physical activity (PA) was assessed using waist worn ActiGraph GT3X accelerometers for a minimum of 5-days (7.6 ± 1.6 days) to obtain average daily steps, sedentary time, and moderate and vigorous PA (MVPA). We measured brachial BP using a SpyghmoCor XCEL after 10-minutes of supine rest. We assessed plasma concentrations of sCD14 using an enzyme-linked immunosorbent assay kit. Normality was assessed using Shapiro-Wilk. Students’ T Test or Mann Whitney test were used to make age comparisons between young (<35 years) and older (>45 years) adults. Pearson’s correlation and Spearman’s rho, controlled for age, body mass index, and sex, were used to assess relations between MVPA and steps with sCD14. Statistical significance was set as p ≤ 0.05 RESULTS: There was not a difference between young and older adults in circulating sCD14 concentration (young: 2348 ± 441 vs. older: 2487 ± 541 pg/ml, p = 0.501). There was not a difference between young and older adults in MVPA (young: 47 ± 25 vs. older: 48 ± 24 min/day, p = 0.873) or average daily steps (young: 7179 ± 3171 vs. older: 7797 ± 3595 steps/day, p = 0.679). There were not associations between sCD14 and daily MVPA (r = -0.176, p = 0.343) or Steps (rho = -0.278, p = 0.130). CONCLUSION: Our preliminary data indicate that there were no age differences in circulating sCD14 and no associations between habitual physical activity and circulating sCD14.

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