Dehydration and Resistance Exercise Increased Muscle and Immune Cell Oxidative Stress in Trained Men

Authors

Casey R. Appell, Texas Tech UniversityFollow
Nigel C. Jiwan, Texas Tech UniversityFollow
Yasuki Sekiguchi, Texas Tech UniversityFollow
Danielle E. Levitt, Texas Tech UniversityFollow
Hui-Ying Luk, Texas Tech UniversityFollow

Abstract

Dehydration induced osmotic stress and resistance exercise (RE) independently increase reactive oxygen species (ROS) could lead to cellular stress in skeletal muscle (SkM) and peripheral blood mononuclear cells (PBMCs). The proper cellular function of SkM and PBMCs is critical to SkM recovery upon damage. Moreover, increased circulatory ROS can stress vascular endothelium, impacting nutrient delivery to tissues. PURPOSE: To examine the combination of dehydration and single RE bout-induced [H₂O₂] in SkM, PBMCs, and in circulation in RE-trained men. METHODS: RE-trained men (n=11, 21±1yr, height: 175.9±6.2cm, body weight: 79.2±12.3kg, 18.4±6.7%fat) completed two identical RE bouts either hydrated (Urine specific gravity [USG] 2O₂] were measured. RESULTS: PO was significantly (p≤0.05) greater in DE (298.6±1.5mmol/kg, p2O₂], no condition x time interaction effect was observed in SkM, PBMCs, or in circulation. [H₂O₂] was greater in the DE vs. EU condition in SkM (DE: 1.17±0.07mM vs. EU: 0.88±0.06mM; pp=0.002). [H₂O₂] increased from PRE (SkM: 0.85±0.07mM, PBMCs: 0.72±0.04mM) to 1hr (SkM: 1.03±0.08mM; p=0.050, PBMCs: 0.94±0.02mM; pppp2O₂]. CONCLUSION: Completing a bout of RE in a dehydrated state resulted in greater intracellular (SkM and PBMCs), but not circulating, ROS than in a hydrated state. This suggests that RE with adequate hydration could reduce intracellular stress. Despite a lack of circulating ROS in young adults, future research is needed to investigate whether individuals who experience chronic hypohydration (e.g., older adults) would have greater intracellular and systemic ROS.

Recommended Citation

Appell, Casey R.; Jiwan, Nigel C.; Sekiguchi, Yasuki; Levitt, Danielle E.; and Luk, Hui-Ying (2024) "Dehydration and Resistance Exercise Increased Muscle and Immune Cell Oxidative Stress in Trained Men," International Journal of Exercise Science: Conference Proceedings: Vol. 2: Iss. 16, Article 47.
Available at: https://digitalcommons.wku.edu/ijesab/vol2/iss16/47