Mixed High-Fat and Stock Diet Caused a Greater Increase in Body Mass than High-Fat Diet Alone
Increased dietary palatability may lead to excess food consumption which in turn causes an excess of caloric intake and weight gain. Although most obesity studies in rodents utilize a high-fat diet, food variation, a combination of different diets for example, may actually increase body weight and fat mass beyond that of solely high-fat feeding. Purpose: To examine the changes in weight gain and body composition in mice that consume either a high-fat diet or a combination of high-fat and stock diet. Methods: 10 CD-1 male mice were randomly assigned to one of two groups based on dietary composition (N=5/group). HF mice consumed a high-fat diet (60% kcal from fat) and HF+Stock mice consumed a 50:50 combination of high-fat and stock diet (13.5% kcal from fat) for 24-weeks. Bi-weekly measurements were made on body composition (lean and fat mass) using an EchoMRI scanner and body weight using a digital scale. Food intake was recorded weekly. Results: HF and HF+Stock gained significant body weight, 41% and 66% respectively, from baseline to week 24 (P<0.001, F12,84=45.483). On average, HF gained 23% lean mass (P<0.001, F10,70=42.276) and 170% fat mass (P<0.001, F10,70=31.873), while HF+Stock gained 27% lean mass and 260% fat mass from baseline. There were no differences between groups for body weight, lean mass, or fat mass. Conclusions: Although not significant, there was a greater increase in body weight, lean and fat mass in mice that consumed a combination of high-fat and stock diets. The results suggest diet variation has a positive effect in inducing obesity in mice. This study is limited in that only five animals were used in each group, thus leading us to believe that increasing the N per group will result in significant differences between groups. Expansion of this pilot study is warranted in the future.
Carpenter, Katie C.; Davidson, Tiffany; and McFarlin, Brian K.
"Mixed High-Fat and Stock Diet Caused a Greater Increase in Body Mass than High-Fat Diet Alone,"
International Journal of Exercise Science: Conference Proceedings: Vol. 6:
1, Article 32.
Available at: https://digitalcommons.wku.edu/ijesab/vol6/iss1/32