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APPLICATION OF A NOVEL COLLECTION OF EXHALED BREATH CONDENSATE TO EXERCISE AND WORK PHYSIOLOGY SETTINGS

Abstract

J.A. Sol, J.C. Quindry, FACSM

University of Montana, Missoula, MT

Collection of exhaled breath condensate (EBC) is a non-invasive method to obtain biosamples from the lower respiratory tract, an approach amenable to exercise and work physiology applications. Commercially available EBC collection products exist; however, single-use collection devices can be cost-prohibitive, and volumes limit the number of biomarkers that can be examined. PURPOSE: To develop a cost-effective, reproducible methodology for obtaining larger volume EBC samples before and after exercise. METHODS: Participants (26 ± 8 yrs.) completed a 10-min collection of breathing through a novel EBC collection device (N-EBC) while seated and wearing a nose clip. After the initial sample was obtained, a 45-min bout of cycling at 60% VO2max was performed, followed by an immediate post-exercise N-EBC sample collection. In a subset of individuals (n=5), EBC was obtained using both the novel technique and a commercially available EBC collection device (R-EBC) in a randomized fashion. RESULTS: N-EBC volume pre- and post-exercise (2.3±0.8 and 2.6±0.9 mL, respectively) and pH (7.4±0.5 and 7.4±0.5, respectively) were not significantly different. N-EBC Salivary α-Amylase values were below the normal range for saliva samples and comparable to R-EBC (0.08±0.56 and 0.21±0.35 U/mL, respectively). No gender differences were observed (males=10; females=6) for N-EBC comparisons. When normalized for participant body height, device comparisons indicated N-EBC volumes were larger than R-EBC at pre-exercise (+12%) and post-exercise (+48%). Pre-exercise pH was significantly different between the N-EBC and R-EBC trials (7.4±0.5 and 6.3±0.2, respectively; p

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