Effects of Manganese Administration on Breathing


1Mohamed, Z., 1Gorczynski, D., 1Glausen, T., 2DeRuisseau K., 1DeRuisseau, L.

1Department of Biological Sciences, Le Moyne College, Syracuse, NY

2Department of Exercise Science, Syracuse University, Syracuse, NY

Purpose: Manganese (Mn) is an important trace element involved with biological functions including macronutrient metabolism and antioxidant defenses. We hypothesized that short term Mn administration would alter the ventilatory response to hypoxia compared to saline administration. Methods: Eleven week old male mice were injected with MnCl2 (intraperitoneal, 12mg/kg body mass; n=6) or saline (n=4) for 7 days followed by unrestrained barometric plethysmography on the 8th day. Mice were acclimated to the chamber and a baseline with room air (BASE) was collected followed by 10 minutes of hypoxia (10% O2, HYP) exposure. Results: Data are presented control vs. experimental and mean ± sem. Breathing frequency (breaths/min, BASE: 101 ± 18 vs. 140 ± 10; HYP: 176 ± 19 vs. 197 ± 12), tidal volume (ml/breath, BASE: 0.26 ± 0.01 vs. 0.35 ± 0.06; HYP: 0.25 ± 0.02 vs. 0.43 ± 0.07), and minute ventilation (ml/min, BASE: 25.8 ± 3.2 vs. 49.8 ± 10.0; HYP: 44.2 ± 5.4 vs. 86.3 ± 18.1) were not different (pE/VCO2 during baseline conditions (BASE: 56.5 ± 5.2 vs. 80.0 ± 17.7). The percent change from baseline approached significance for tidal volume (control vs. experimental; 0.25 vs. 0.43; p=0.08). Conclusions: These preliminary findings indicate that MnCl2 does not alter the ventilatory response during baseline, although the tidal volume percentage change may reveal differences as the study is expanded.

Funding provided by: Le Moyne College Student Research Committee, Biological Sciences

Department, and American Physiological Society STRIDE Program; ZM is a 2013 STRIDE Fellow.

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