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Effect of Bisphenol A (BPA) on Skeletal Muscle Oxidative Stress

Abstract

Receno, C., Benson, M., Liang, C., Keslacy, S., DeRuisseau, K. Syracuse University, Syracuse, NY

Purpose: Bisphenol A (BPA) is a chemical used in many plastics and food-contact materials that has been linked to several health conditions, including type II diabetes. BPA is classified as an “endocrine disruptor” for its potential to exert estrogen-like activity and has been linked to the development of oxidative stress. BPA exposure may be particularly important to skeletal muscle since changes in redox status can have large implications for contractile and metabolic properties. Therefore, the aim of this study was to determine the impact of BPA exposure on indices of oxidative stress in skeletal muscle. Methods: Mouse myoblast C2C12 cells were cultured and exposed to BPA or a 0.1% ethanol vehicle. The BPA concentrations examined included 1nM, 10nM or 100nM for incubation times of 24 hours, 6 hours, 40 minutes or 15 minutes. Triplicate samples within each experiment were pooled, and each experiment was conducted three times. Using the Western Blot technique, samples were analyzed for oxidant levels using 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) antibodies as well as key antioxidant enzymes; superoxide dismutase-1 (SOD1) and glutathione peroxidase (GPX). Data were analyzed via 2-way or 1-way ANOVAs with SPSS v21. Results: No significant differences were detected in dose/exposure groups for oxidant levels (3-NT and 4-HNE) or GPX. However, at 24 hours, 100 nM BPA resulted in greater SOD1 (11.28+2.8 fold change, pConclusion:BPA exposure for 24 hours at 100nM concentration elevated SOD1 levels compared to all other time points and doses but did not increase markers of oxidant injury. These data suggests that chronic long term BPA exposure could alter the skeletal muscle antioxidant status.

Research funded by Syracuse University, School of Education.

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