The platinum compound Cl [Chloro[N,N-diethyldiethylenetriamine] Platinum(II) Chloride] was synthesized and reacted with N-acetylmethionine (N-AcMet) and guanosine 5’-monophosphate (5’-GMP). Previous experiments show that N-AcMet reacts kinetically faster with the central platinum atom and that 5’-GMP bonds slower yet stronger. When Cl was reacted with N-AcMet the N-AcMet displaced the chloride ion as expected. When Cl was reacted with 5’-GMP the 5’-GMP binds to the chloride ion and the reaction is finished. Yet, when 5’-GMP was added in the solution already containing N-AcMet, the N-AcMet was not displaced as predicted. Through 1H NMR Spectroscopy and Mass Spectrometry we observed that the 5’-GMP also bonds to the central platinum ion displacing one of the nitrogen atoms of the chelate. Multiple experiments were done adding different ratios of N-AcMet and 5’-GMP and at different stages of the reaction. It was observed that the dominant product is the same, and the reaction is complete in approximately 24 hours. Platinum compounds similar to , but with varying degrees of bulk surrounding the central molecule, have been reacted with N-AcMet and 5’-GMP, yet the formation of an analogous product was not observed.
Advisor(s) or Committee Chair
Dr. Kevin Williams
Gruner, Morgan, "The Reaction of Platinum Triamine Complex with Different DNA and Protein Complexes" (2014). Honors College Capstone Experience/Thesis Projects. Paper 465.