Advisor(s) - Committee Chair
James Skean, Frank Toman, F. Morgado
Department of Biology
Master of Science
Variously aged (C57BL X C3H)F1 hybrid mice were used as recipients in experiments to determine if spleen or bone marrow cells from normal C57BL or C3H donors, spleen or bone marrow cells from C57BL donors previously treated with cortisone, or C57BL bone marrow cells previously incubated with serum from X-irradiated C57BL mice could induce a graft-versus-host reaction (GVHR).
The mice were assayed for GVHR by mortality or splenomegaly as detected by the discriminant spleen assay developed by Simonsen. Results show that spleen cells from normal C57BL, normal C3H, or cortisone-treated C57BL mice are capable of inducing a GVHR in F1 hybrids, resulting in death or splenomegaly, whereas bone marrow cells from normal C3H, normal C57BL, or cortisone-treated C57BL mice were unable to induce a GVHR in F1 hybrids. It was also found that sera from lethally irradiated C57BL mice did not cause C57BL bone marrow to induce a GVHR in F1 hybrids.
Biology | Cell and Developmental Biology | Cell Biology | Life Sciences
Grane, Ronald, "The Effect of Parental Bone Marrow & Spleen Cells in the Induction of the Graft-Versus-Host Reaction" (1978). Masters Theses & Specialist Projects. Paper 2405.