Publication Date


Advisor(s) - Committee Chair

James Skean, Frank Toman, F. Morgado

Degree Program

Department of Biology

Degree Type

Master of Science


Variously aged (C57BL X C3H)F1 hybrid mice were used as recipients in experiments to determine if spleen or bone marrow cells from normal C57BL or C3H donors, spleen or bone marrow cells from C57BL donors previously treated with cortisone, or C57BL bone marrow cells previously incubated with serum from X-irradiated C57BL mice could induce a graft-versus-host reaction (GVHR).

The mice were assayed for GVHR by mortality or splenomegaly as detected by the discriminant spleen assay developed by Simonsen. Results show that spleen cells from normal C57BL, normal C3H, or cortisone-treated C57BL mice are capable of inducing a GVHR in F1 hybrids, resulting in death or splenomegaly, whereas bone marrow cells from normal C3H, normal C57BL, or cortisone-treated C57BL mice were unable to induce a GVHR in F1 hybrids. It was also found that sera from lethally irradiated C57BL mice did not cause C57BL bone marrow to induce a GVHR in F1 hybrids.


Biology | Cell and Developmental Biology | Cell Biology | Life Sciences