Publication Date


Advisor(s) - Committee Chair

Larry Elliott, Martin Houston, Thomas Coohill

Degree Program

Department of Biology

Degree Type

Master of Science


The activation of the precarcinogen 2-aminoanthracene was investigated employing cell-free extracts of Escherichia coil, Pseudomonas aeruginosa, Streptococcus faecalis, Bacteroides fragilis, Clostridium perfringens and the eukaryote Candida albicans. A modification of the Salmonella typhimurium/mammalian microsomal mutagenicity assay was employed to determine activation. Of the species tested, only cell-free extracts of Escherichia coli and Bacteroides fragilis failed to induce the reversion of the Salmonella typhimurium his- mutants TA-98 and/or TA-100 to his+ revertants.

Separation of the cytosol and membrane pellet enzymes was completed. Of the organisms tested only the membrane pellet enzymes of Clostridium perfringens showed activation of 2-aminoanthracene.


Biology | Life Sciences

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