Advisor(s) - Committee Chair
Valgene Dunham, Frank Toman, Martin Houston
Department of Biology
Master of Science
DNA polymerase alpha, the enzyme involved in nuclear DMA replication, and DNA polymerase gamma, the enzyme involved in organellular DNA replication, were isolated and purified from soybean and turnip. The enzymes were characterized following ammonium sulfate precipitation, DEAF-cellulcse, phosphocelluloca, and hydroxylapatite chromatography, and by non-denaturing polyacrylamide gel electrophoresis. Protein banct were electropluted and the enzymes characterized using kinetic studies and sensitivity to divalent cations and inhibitors. Molecular weight and subunit composition studies indicated a molecular weight for the catalytic subunit of DNA polymerase alpha in soybean and turnip to be 46kDa. DNA polymeraqe gamma was composed of a catalytic subunit with a molecular weight of 66kDa. Although the two enzymes appear to share common subunits, characterization of their genetic origin remains to be determined before alpha and gamma can be classified as isoenzymes.
Biology | Life Sciences
Hill, Donna, "Isolation & Characterization of DNA Polymerase Alpha and Gamma from Turnips (Brassica rapa) and Etiolated Soybeans (Glycine max)" (1988). Masters Theses & Specialist Projects. Paper 2483.