Publication Date


Advisor(s) - Committee Chair

Larry Elliott, Lewis Lockwood, Martin Houston

Degree Program

Department of Biology

Degree Type

Master of Science


Tartaric acid accumulation in fermentation broth of Gluconobacter suboxydans was investigated qualitatively with paper chromatography and thin-layer chromatography. Comparisons were made using six different solvent systems and four different chemical indicators commonly used in chromatography.

Attempts were made to establish a routine method for quantitative analysis of tartaric acid. Spectrophotometry was used to detect possible metabolites resulting from glucose oxidation by G. suboxydans in different analytical separations. A combined method of ion-exchange column chromatography and paper chromatography followed by ammonium meta-vanadate spectrophotometric analysis differentiated 5-oxogluconic acid and tartaric acid and thus gave better quantitative results in the determination of tartaric acid from fermentation broths.


Biology | Life Sciences

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