Publication Date


Advisor(s) - Committee Chair

Martin Houston, Lewis Lockwood, David Hartman

Degree Program

Department of Biology

Degree Type

Master of Science


NADP+-dependent 5-ketogluconate reductase (D-Gluconate:NADP+ oxidoreductase; E.C.I.I.I.69.) of Gluconoacter suboxydans was examined and determined to be constitutive in nature. Crude, cell -free enzyme extracts were prepared by sonication of cells grown on 2.0'% glucose and 2.0% ethanol in various experiments. The specific activity of 5-ketogluconate reductase in the crude enzyme extracts of cells grown under various conditions was determined. The specific activity remained relatively constant strongly indicating the constitutive nature of the enzyme.


Biology | Life Sciences

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