Publication Date


Advisor(s) - Committee Chair

Valgene Dunham, Frank Toman, Martin Houston

Degree Program

Department of Biology

Degree Type

Master of Science


Two forms of glutamine synthetase (GS) have been isolated from three-day old etiolated soybean hypocotyls by ammonium sulfate precipitation and DEAE-cellulose chromatography. The GS isoforms were eluted from a 30 ml DEAE-cellulose column using 0.14 M KC1 and 0.175 M KC1 and from a 1.0 ml DEAE-cellulose column using 0.08 M KC1 and 0.14 M KC1. The two isoforms of soybean GS have a molecular weight of about 392,000 daltons as indicated by in gel enzyme assays and staining of protein bands.

The poly(A)+RNA specific for GS synthesis was isolated using phenol and chloroform followed by Hybond mAP paper. Translation was performed using a wheatgerm cell-free lysate. Following the application of the in vitro translated products to a 1 ml DEAE-cellulose column, GS was successfully eluted at 0.14 M KC1.


Biology | Life Sciences

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