Circulating Lipid Mediators Modulate Skeletal Muscle Repair

Authors

Logan Moore, Texas Wesleyan UniversityFollow
Kamal Awad, University of Texas at ArlingtonFollow
Marco Brotto, University of Texas, ArlingtonFollow

Abstract

Skeletal muscle injuries account for the majority of sports related trauma. However, skeletal muscle has a unique quality in its’ innate ability to repair and regenerate. PURPOSE: The goal of this study was to detail the regenerative capacity of skeletal muscle through analysis of lipid mediator (LM) signaling in the circulation. METHODS: Wild-type (C57BL/6) mice were blindly randomized into 4 groups: Baseline-Control, 4-Day post injection, 7-Day, and 1-month (n=12). Skeletal muscle damage was initiated through an intra-muscular injection of 50 μL of 1.2% Barium Chloride (BaCl₂) in the tibialis anterior (TA). The contralateral limb served as the sham control injected with saline. The TA was embedded in paraffin and transversely cut for histological analysis. Serum was processed for targeted lipidomics following our own LC-MS/MS methodology. Data was analyzed for variance using One-way ANOVA. RESULTS: H&E histological assessment confirmed acute skeletal muscle damage and regeneration via injection of BaCl₂. At 4-Days post – injection there is an influx of mononuclear monocytes and the clearance of necrotizing myofibers. Congruently, there is a significant increase in Palmitoylethanolamide (PEA) in serum (pCONCLUSION: The remarkable regenerative capacity of skeletal muscle might be influenced by specific lipid mediators in serum after damage of skeletal and during the period post-damage.

Recommended Citation

Moore, Logan; Awad, Kamal; and Brotto, Marco (2025) "Circulating Lipid Mediators Modulate Skeletal Muscle Repair," International Journal of Exercise Science: Conference Proceedings: Vol. 2: Iss. 17, Article 147.
Available at: https://digitalcommons.wku.edu/ijesab/vol2/iss17/147