Advisor(s) - Committee Chair
Martin Houston, Claire Rinehart, Frank Toman, David Hartman
Department of Biology
Master of Science
Hemoglobin Zurich is a rare blood condition which causes severe rupturing of erythrocytes upon the administration of sulfonamides. Hemoglobin Zurich is caused by a specific genetic mutation in which there is a single amino acid (histidine) substituted for arginine at position 63 in the beta chain of hemoglobin molecules [β 63 Histidine --> Arginine].
In this study, the membrane proteins of Hemoglobin Zurich erythrocytes were compared to membrane proteins from normal human erythrocytes employing sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and visualized by periodic-acid Schiff (PAS) and DZANDU staining. No differences were detected employing these techniques.
DNA was isolated from leucocytes of a Hemoglobin Zurich individual, amplified and subsequently seque-Iced by the dideoxynucleotide method. The actual mutation was identified as a substitution of a G at base 188 of the coding region for an A.
Biology | Life Sciences
Liu, Yijuan, "A Comparison of Hemoglobin Zurich Erythrocytic Membrane Proteins & Identification of the Mutation at the DNA Level" (1993). Masters Theses & Specialist Projects. Paper 2534.