My goals as proposed were to: 1) increase resolution and support for a chloroplast DNA phylogeny by acquiring more sequence data, 2) generate a robust nuclear DNA phylogeny by sequencing several single-copy genes, 3) use flow cytometry to estimate DNA quantity per cell (i.e., ploidy level) in R. ursinus and NCGR specimens of unknown ploidy level, 4) identify highly variable DNA microsatellite (or simple-sequence repeats) regions to assess genetic diversity in R. bartonianus and test species boundaries, and 5) prepare and submit manuscripts if sufficient data have been generated.
Biology | Cell and Developmental Biology | Plant Biology
Alice, Lawrence Alan, "[Sabbatical Report]" (2014). Sabbatical Reports. Paper 15.