Publication Date

6-1981

Advisor(s) - Committee Chair

Rebecca Brown, Martin Houston, Rodney McCurry, Eugene Hoffman

Degree Program

Department of Biology

Degree Type

Master of Science

Abstract

One strain of Bacillus subtilis and three antibiotic-resistant strains of Staphylococcus aureus were employed to determine the biological effects of everninomicin B (EvB), a naturally occurring antibiotic produced by Micromonospora carbonacea (NRRL 2972) and M. carbonacea var. aurantiaca. Minimum inhibitory concentration (MIC) of EvB for B. subtilis was 1.2 x 10-3 µmole/ml of EvB in glucose minimal broth and 2.6 x 10-3 µmole/ml of EvB in nutrient broth. MIC values of EvB for S. aureus were 3.25 x 10-4 µmole/ml of EvB for strains resistant to penicillin or tetracycline and 6.5 x 10-4 µmole/ml of EvB for the aminoglycoside-resistant strain. The inhibitory effect of EvB was found to be reversible for all concentrations of EvB and all bacterial strains tested. The inhibitory effect of EvB for B. subtilis was not dependent upon the initial concentration of cells nor the stage in the growth cycle at the time the compound was introduced. The inhibitory effect was dependent upon the initial concentration of cells for S. aureus. Electron microscopy studies showed distinct morphological changes in treated cells of S. aureus. Cellular lysis in these cells was also detected.

Disciplines

Biology | Immunology and Infectious Disease | Immunology of Infectious Disease | Life Sciences

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