Publication Date

7-1976

Advisor(s) - Committee Chair

Thomas Coohill, David Hartman, Scott Ford

Degree Program

Department of Biology

Degree Type

Master of Science

Abstract

For our investigation, an established line (E line) of baby hamster kidney cells which was transformed by the incorporation of Simian Virus 40 into the cellular genom was used. The induction of this virion by irradiating these cells with ultraviolet light was studied. The purpose of this thesis was to determine the wavelength dependency (at four selected ultraviolet mercury emission lines) of the induction of the SV40 genome from these cells. The viral titres of induction were measured by the agar overlay plaque assay method and by the fluorescent antibody microassay technique.

At the four wavelengths tested 265 nm and 280 nm were approximately equal in their ability to induce the virus from the cells. Light at 240 nm was less effective than either of these two wavelengths in inducing virus production. The 297 nm wavelength proved to be the least effective of all four wavelengtns tested.

Disciplines

Animal Sciences | Biology | Life Sciences

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