Publication Date
Spring 2018
Advisor(s) - Committee Chair
Dr. Noah Ashley (Director), Dr. Claire Rinehart, and Dr. Cheryl Davis
Degree Program
Department of Biology
Degree Type
Master of Science
Abstract
Despite the convergence of rapid technological advances in genomics and the maturing field of ecoimmunology, our understanding of the genes that regulate immunity in wild populations is still nascent. Previous work to assess immune function has relied upon relatively crude measures of immunocompetence. However, with next-generation RNA-sequencing, it is now possible to create a profile of gene expression in response to an immune challenge. In this study, captive zebra finch (Taeniopygia guttata; adult males) were challenged with bacterial lipopolysaccharide (2 mg/Kg BW; dissolved in 0.9% saline) or vehicle (0.9% saline) to stimulate the immune system. Two hours after injection, birds were euthanized and hypothalami, spleen, and red blood cells (RBCs) were collected. Taking advantage of the fully sequenced genome of zebra finch, total RNA was isolated, sequenced, and partially annotated in these tissue/cells. The data show 628 significantly upregulated transcripts in the hypothalamus, as well as 439 and 121 in the spleen and RBCs, respectively, relative to controls. Also, 134 transcripts in the hypothalamus, 517 in the spleen, and 61 in the RBCs were significantly downregulated. More specifically, a number of immunity-related transcripts (e.g., IL-1β, RSAD2, SOCS3) were upregulated among tissues/cells. Additionally, transcripts involved in metabolic processes (APOD, LRAT, RBP4) were downregulated, suggesting a potential trade-off in expression of genes that regulate immunity and metabolism. Unlike mammals, birds have nucleated RBCs, and these results suggest a novel transcriptomic response of RBCs to immune challenge. Lastly, molecular biomarkers could be developed to rapidly screen bird populations by simple blood sampling in the field.
Disciplines
Genetics and Genomics | Immunity | Ornithology
Recommended Citation
Scalf, Cassandra, "Transcriptomic Response to Immune Challenge in Zebra Finch (Taeniopygia Guttata) Using RNA-SEQ" (2018). Masters Theses & Specialist Projects. Paper 2341.
https://digitalcommons.wku.edu/theses/2341
Included in
Genetics and Genomics Commons, Immunity Commons, Ornithology Commons