Publication Date
3-1984
Advisor(s) - Committee Chair
Larry Elliott, Martin Houston, Thomas Coohill
Degree Program
Department of Biology
Degree Type
Master of Science
Abstract
The activation of the precarcinogen 2-aminoanthracene was investigated employing cell-free extracts of Escherichia coil, Pseudomonas aeruginosa, Streptococcus faecalis, Bacteroides fragilis, Clostridium perfringens and the eukaryote Candida albicans. A modification of the Salmonella typhimurium/mammalian microsomal mutagenicity assay was employed to determine activation. Of the species tested, only cell-free extracts of Escherichia coli and Bacteroides fragilis failed to induce the reversion of the Salmonella typhimurium his- mutants TA-98 and/or TA-100 to his+ revertants.
Separation of the cytosol and membrane pellet enzymes was completed. Of the organisms tested only the membrane pellet enzymes of Clostridium perfringens showed activation of 2-aminoanthracene.
Disciplines
Biology | Life Sciences
Recommended Citation
Hamill, Thomas, "Activation of 2-Aminoanthracene by Cell-Free Microbial Extracts" (1984). Masters Theses & Specialist Projects. Paper 2421.
https://digitalcommons.wku.edu/theses/2421