Publication Date

7-1976

Advisor(s) - Committee Chair

Martin Houston, Lewis Lockwood, David Hartman

Degree Program

Department of Biology

Degree Type

Master of Science

Abstract

NADP+-dependent 5-ketogluconate reductase (D-Gluconate:NADP+ oxidoreductase; E.C.I.I.I.69.) of Gluconoacter suboxydans was examined and determined to be constitutive in nature. Crude, cell -free enzyme extracts were prepared by sonication of cells grown on 2.0'% glucose and 2.0% ethanol in various experiments. The specific activity of 5-ketogluconate reductase in the crude enzyme extracts of cells grown under various conditions was determined. The specific activity remained relatively constant strongly indicating the constitutive nature of the enzyme.

Disciplines

Biology | Life Sciences

Included in

Biology Commons

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