Publication Date

8-1984

Advisor(s) - Committee Chair

Frank Toman, Larry Elliott, Martin Houston

Degree Program

Department of Biology

Degree Type

Master of Science

Abstract

Human chorionic gonadotropin (hCG), labeled with 125I, was used to study the binding properties of hCG to polystyrene beads. The binding capacity of the 0.25-in diameter beads was determined to be approximately 300 mIU/bead. The affinity constant, K, was determined to be essentially O, suggesting that binding is strictly passive. The binding of hCG to polystyrene was proportional to the hCG concentration and temperature. There were highly significant (p<0.001) differences among amounts of hCG bound at 3 C, 25 C and 37 C. There was a highly significant (p<0.001) relationship between the amount of hCG bound and the initial hCG concentration. There did not appear to be any correlation between the percentage of hCG bound and the initial hCG concentration. Attempts to use Staphylococcal Protein A-peroxidase conjugate to detect murine monoclonal antibodies to beta-hCG were not successful.

Disciplines

Biology | Life Sciences

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