Xiaohong Jin

Publication Date

8-1998

Advisor(s) - Committee Chair

Eric Conte, John Riley, Wei-Ping Pan

Comments

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Degree Program

Department of Chemistry

Degree Type

Master of Science

Abstract

Most cloud-point extraction procedures for High Pressure Liquid Chromatography are limited to applications requiring a detector other than the most commonly used UV/Vis detector. The high background created by the injected aliquot of the surfactant-rich extract makes trace analysis impossible. Cetrimide, an cationic surfactant mixture consisting of mainly tetradecyltrimethylammonium bromide with lesser amounts of C16 and C12 homologs, was used in this research. Components in Cetrimide do not absorb strongly in UV/Vis region, making it an ideal cloud point surfactant candidate for use with the UV/Vis detector.

The extraction of selected chlorophenols in drinking water was applied to test the effectiveness of this procedure. Cetrimide solution was added to a sample of drinking water containing chlorophenols. The sample was then shaken. Sodium chloride was added resulting in a cloud formation at ambient conditions. Before centrifugation, a co-surfactant (1-octanol) was added to aid in the separation of surfactant-rich and aqueous phases. An aliquot of the surfactant-rich phase was then injected into a HPLC with UV/Vis detection. Recoveries greater than 90% and detection limits lower than 70 parts-billion for the selected chlorophenols were obtained with this procedure. The ionic surfactant cloud-point procedure presented provides a novel alternative to other techniques of preconcentration and separation for use with HPLC UV/Vis detection.

Disciplines

Chemistry | Physical Sciences and Mathematics

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